By Gerald Thouand, Robert S. Marks

This e-book describes the layout and using bioluminescent biosensors. It introduces rookies and skilled researchers beginning within the microbiological biosensor area to the sensible elements of creating a luminescent microbial biosensor. it's also a resource of data approximately different purposes that use microbial cells. each one bankruptcy focuses so far as attainable at the technological perception of the awarded biosensor with a transparent demonstration of the problems within the layout and the way to arrive the facts of proposal. The booklet is split into 3 useful sections facilitating the reader to simply entry the knowledge, ranging from the bioreporter dealing with (free, immobilized, or spore) to the engineering of the size platform (fiber optic, CCD, lensless platform, free-cell bioreactor, CD platform).

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1 μg/L [53] Mercury E. 9 ppm Naphthalene Pseudomonas putida 50 nM [26] Cooper Alcaligenes eutrophus 1 μM [55] Tributyltin E. coli 0,02 μM [56] Methyl parathion Flavobacterium 0,3 μM [22] © 2016 by Taylor & Francis Group, LLC July 29, 2015 13:19 PSP Book - 9in x 6in 03-Gerald-Thouand-c03 Fiber Optic Whole-Cell-Based Biosensors water) are required; there two main preparation and measuring methodologies, water–soil-mixed solution or supernatant of the soil–/sediment–water suspension. A biosensor for detecting the toxicity of phenanthrene (polycylic aromatic hydrocarbon [PAH])-contaminated soil has been successfully constructed using an agar-immobilized recombinant bioluminescent bacterium, GC2 (lac::luxCDABE), which constitutively produces bioluminescence [51].

Rogers, K. R. (1998). Enzyme and Microbial Biosensors: Techniques and Protocols, Humana Press, Totowa, NJ, USA. 26. , Durand, M. , Thouand, G. (2011). A multi-channel bioluminescent bacterial biosensor for the on-line detection of metals and toxicity. Part I: design and optimization of bioluminescent bacterial strains. Anal. Bioanal. , 400, 1051–1060. 27. , Durand, M. , Thouand, G. (2007). Technological conception of an optical biosensor with a disposable card for use with bioluminescent bacteria.

15. , Thouand, G. (2009). Properties and choice of material used for microbial biosensor. In Biosensors: Properties, Materials and Applications, Rafael Comeaux and Pablo Novotny, New York. USA, pp. 87–126. 16. Ball, P. (1999). Applications of microporous membranes in sensor technology. Sens. , 19, 46–51. 17. Roach, P. , Ramsden, D. , Williams, P. (2003). Development of a conductimetric biosensor using immobilised Rhodococcus ruber whole cells for the detection and quantification of acrylonitrile.

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Bioluminescent Microbial Biosensors: Design, Construction, by Gerald Thouand, Robert S. Marks
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