By Jonathan Y. Richmond, Robert W. McKinney

Describes the mixtures of normal & distinctive microbiological practices, security equip., & amenities that are urged for paintings with numerous infectious brokers in numerous labs. This revision takes under consideration such concerns as worldwide predicament approximately rising & reemerging infectious illnesses; elevated curiosity in prion illnesses with the identity of BSE in England; the prevalence of a number of lab-associated infections; elevated main issue in regards to the nationwide & intl. move of infectious microorganisms; & growing to be matters approximately bioterrorism, & the elevated defense wishes of our microbiological labs.

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B. Special Practices None 18 Laboratory Biosafety Level Criteria – Biosafety Level 1 C. Safety Equipment (Primary Barriers) 19 Laboratory Biosafety Level Criteria – Biosafety Level 2 1. Special containment devices or equipment such as a biological safety cabinet are generally not required for manipulations of agents assigned to Biosafety Level 1. 2. It is rec om me nde d tha t labo rator y coat s, go wns , or un iforms be worn to prevent contamination or soiling of street clothes. 3. Gloves should be worn if the skin on the hands is broken or if a rash is present.

2. Policies for safe handling of sharps are instituted. 3. All procedures are performed carefully to minimize the creation o f aeroso ls. 4. Work surfaces are decontaminated at least once a day and afte r any spill of viable mate rial. 5. All waste is decontaminated before disposal by an approved method such as autoclaving. 6. An insect and rodent control program is in effect (see Appendix G). B. Special Practices 1. Only persons whose presence in the facility or individual laboratory rooms is required for program or support purposes are authorized to enter.

Filters must be repla ced as ne ede d. An altern ative is to us e por table vacuum pumps (also properly protected with traps and filters). 13. An eyewash station is readily available inside the laborator y. 14. Illumination is adequate for all activities, avoiding reflections and glare that could imped e vision. 15. The Biosafety Level 3 facility design and operational procedures must be documented. The facility must be 36 Laboratory Biosafety Level Criteria – Biosafety Level 4 tested for verification that the design and operational parameters have been met prior to operation.

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Biosafety in Microbiological and Biomedical Laboratories by Jonathan Y. Richmond, Robert W. McKinney
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