By Shan S. Wong

Since the book of the 1st version of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, significantly multifunctional cross-linkers, were constructed and synthesized. The of completion of the human genome undertaking has opened a brand new region for learning nucleic acid and protein interactions utilizing nucleic acid cross-linking reagents, and advances have additionally been made within the region of biosensors and microarray biochips for the detection and research of genes, proteins, and carbohydrates. additionally, advancements in actual thoughts with extraordinary sensitivity and backbone have facilitated the research of cross-linked items.

Updated to mirror the advances of the twenty first century, this publication offers:

  • An assessment of the chemical rules underlying the methods of cross-linking and conjugation
  • A thorough checklist of cross-linking reagents released within the literature because the first variation, masking monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers
  • Reviews of using those reagents in learning protein tertiary constructions, geometric preparations of subunits inside of complicated proteins and nucleic acids, near-neighbor research, protein-to-protein or ligand–receptor interactions, and conformational alterations of biomolecules
  • Discusses the appliance of immunoconjugation for immunoassays, immunotoxins for special treatment, microarray know-how for research of varied biomolecules, and sturdy country chemistry for immobilizations

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Chemistry of Protein and Nucleic Acid Cross-Linking and by Shan S. Wong
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