By Knight / Wolstenholme Editors
Chapter 1 Chairman's creation: technique of the Viral Genome (pages 1–4): J. H. Subak?Sharpe
Chapter 2 a few Enzymes laid out in DNA Phages (pages 5–24): Seymour S. Cohen
Chapter three Enzymes laid out in DNA?Containing Animal Viruses (pages 25–44): B. R. Mcauslan
Chapter four Extracellular thoughts of a Replicating RNA Genome (pages 45–73): S. Spisgelman
Chapter five extra Characterization of the Genetic Map of Poliovirus Temperature?Sensitive Mutants (pages 75–100): P. D. Cooper, E. Geissler, P. D. Scotti and G. A. Tannock
Chapter 6 The Synthesis of Protein through Mammalian RNA Viruses (pages 101–110): David Baltimore, Alice Huang, Kenneth F. Manly, Dawd Rekosh and Martha Stampfer
Chapter 7 the interpretation and Processing of Poliovirus Proteins (pages 111–140): D.F. Summers, M. Roumiantzeff and J. V. Maizel
Chapter eight Phage Repressors (pages 141–153): Mark Ptashne
Chapter nine The Subversion of the Bacterial Transcription equipment in the course of Phage an infection (pages 155–168): Andrew Travers
Chapter 10 Phage?Specified move RNA's (pages 169–178): Urn Z. Littauer, Violet Daniel and Sara Sarid
Chapter eleven Transcription in vitro of the Eschenchia coli tRNATyr Gene Carried by way of the Transducing Bacteriophage 80PSU+3 (pages 179–189): Uribl Z. Littauer, Violet Daniel, Jacques S. Beckmann and Sara Sarid
Chapter 12 Phage?Induced Conversion of Host Valy?tRNA Synthetase (pages 191–205): William H. Mcclain, George L. Marchin and Frederick C. Neidhardt
Chapter thirteen Lambda keep an eye on platforms (pages 207–228): Francois Gros, Nat Sternberg, Marcel Bouquet and Philippe Kourilsky
Chapter 14 technique of Simian Virus forty (pages 229–265): Saul package, D. R. Dubbs and ok. Somers
Chapter 15 Polyoma Gene features Required for mobile Transformation (pages 267–274): Walter Eckhart
Chapter sixteen Temperature?Restricted Mutants of Human Adenovirus kind five (pages 275–294): J. F. Williams and S. Ustacelebi
Chapter 17 Virus?Induced, Tumour?Associated Antigens (pages 295–315): George Klein
Chapter 18 The RNA Tumour Virus DNA Polymerase: research of the Endogenous and Exogenous Reactions (pages 317–323): David Baltimore, Donna Smoler, Kenneth F. Manly and Esther Bromfeld
Chapter 19 Proteins laid out in RNA Tumour Viruses (pages 325–334): S. Spiegelman
Chapter 20 Adenovirus?Specified Proteins (pages 335–353): W. C. Russell
Chapter 21 Herpes Virus?Specified Proteins (pages 355–387): J. Hay, A. J. Perera, J. M. Morrison, G. A. Gentry and J. H. Subak?Sharpe
Chapter 22 Chairman's Conclusions: technique of the Viral Genome (pages 389–394): J. H. Subak?Sharpe
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Additional info for Ciba Foundation Symposium - Strategy of the Viral Genome
R. MCAUSLAN Roche Institute of Molecular Biology, Nutley, New Jersey THEfield of bacteriophage-specified enzymes, now large enough for a whole book (Cohen 1968), provided the stimulus for a corresponding study on animal viruses, for it became of interest to know whether animal viruses whose DNA molecules are of the order of 20-2oox I O ~daltons carry information for setting up enzymic systems for the synthesis of their own DNA. Compared to the bacteriophage systems the list of enzymes elicited by animal deoxyviruses is remarkably small : DNA polymerase, RNA polymerases, thymidine kinase, ligase, ATPase and a few deoxyribonucleases constitute virtually the whole catalogue (for recent reviews see Kit 1968; Black 1968; McAuslan 1970).
And WHSSBACH, A. (1969)J. Virol. 4, 15-23. BWS, K. , SILVERMAN, BLACK, P. H. (1968) A. Rev. Minobiol. 22,391. CHANG, L. M. and HODES, M. E. (1967) Virology 32,258-266. COHEN,S. (1968) Virus-induced Enzymes. New York: Columbia University Press. DAHL,R. and KATES,J. R. (1970) Virology 4,463-472. ERON,L. and MCAUSLAN, B. R. (1966) Biochem. biophys. Res. Commun. 22, 518-523. GOLD,P. and DALES, S. (1968) Proc. nutn. Acud. Sci. A. 60,845-852. , CAME,P. E. and BREEZE, D. (1966) Virology 29,133-148. JOKLIK, W.
The precipitate was washed onto a Millipore filter and washed fivetimes with ~omlofcold~ o p ecent r TCAcontaining o'gpercent ofNa-pyrophosphate. The Millipore membrane was then dried and counted in a liquid scintillationcounter. m. ). DNase omitted from assay mixture. * responses of the MSz replicase to the various kinds of nucleic acids revealed a striking preference for its own RNA. No significant response was observed with the tRNA or the ribosomal RNA of the host cell. It would appear that our intuitive guess was confirmed.
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