By Malcolm R. Macnaughton (auth.), W. Henle, P. H. Hofschneider, H. Koprowski, F. Melchers, R. Rott, H. G. Schweiger, P. K. Vogt (eds.)
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Extra resources for Current Topics in Microbiology and Immunology
SequenDNA circles ces in the inwith twice the length of verted terminal repetithe linear duplex tions were reported to be 2. Heteroduplex analysis transcribed using terminal HindIII restriction endonuclease fragments of terminally nicked vacciniaDNA also used to show termiI nal repetition Structural features observed Poxvirus DNA 47 ... , 8 7 B 8 7 6 6 5 5 4 4 3 3 2 T €. 1 0 )( ~ 8 v 7 6 4 5 4 3 2 0 10 20 30 o 10 20 30 FRACTION Fig. 3A-D. Sedimentation analysis of vaccinia virus DNA. Virions were purified by method I (with freeze-thawing and sonication) or method II (no freeze-thawing, no sonication) (Holowczak 1976).
1971) As per Shadowing HindIII endonuclease fragments of vaccinia DNA None employed, reference made to size of terminal Internal DNA standard Spreading and preparation of DNA for electron microscopy Methods used to prepare DNA 1. 12 pm determined Intact, fuJI-length molecules Histogram summarizing results presented Size distribution in total population Contour length measurements Estimated mol. wt of intact genome None given, Mol. wt. 8xl06 daltons or 11% of genome involved in these structures Conversion constant mass/micron oflength described Comments 1.
Wt. of intact genome Linear, dsDNA Linear, dsDNA 1. Large population of "halF molecules observ- 1. Complete spreading of all molecules only observed with Method B 1. Molecules not well spread and appear to be associated with protein 1. Author suggests that molecules as observed may have propensity to circularize dsDNA some associated proteins Coiled, dsDNA Comments Structural features observed Detergent lysis in presence of pronase or proteinase K 1. No sonication or freeze-thawing during virus purification 2.
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