By Bernard Roizman (auth.), W. Arber, W. Braun, F. Cramer, R. Haas, W. Henle, P. H. Hofschneider, N. K. Jerne, P. Koldovsky, H. Koprowski, O. Maaløe, R. Rott, H.-G. Schweiger, M. Sela, L. Syruček, P. K. Vogt, E. Wecker (eds.)
"When we supply a definition it's for the aim of utilizing it". HENRI POINCARE in technological know-how and approach A. targets the 1st model of this paper was once written to introduce new scholars and fellows of my laboratory to the mysteries of herpesviruses. Consonant with this layout sections facing good documented facts have been trimmed to the bone while many vague phenomena, arguable info and possible trivial observations have been mentioned generously and at size. there's a few doubt to whether it was once intended to be released, however it used to be now not a assessment. the target of studies is often to carry order. yet regrettably, even the main fluent summation of credible information often makes boring examining and an excessive amount of believable order, like little or no entropy in chemical reactions, isn't the best suited setting on which to nurture the urge to find. This model is extra charitable yet now not much less inbalanced. The bibliography displays the motive of the paper and used to be up to date final in December of 1968. it may be visible with no asserting that no unmarried account corresponding to this may do justice or injustice, because the case might be, to different hundred papers released on herpesviruses every year or to the numerous thousand papers released on herpesviruses because the first of the family members was once experimentally transmitted to a heterologous host greater than part a century in the past (GRUTER, 1924). B. Definition 1.
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Additional resources for Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitatsforschung
I, ~S I 14C 1\ I / . ~'... -I -;;; .. Cfok- 0 :. >::~! ~ I I .. _? :63H OK ~OO OK O~~~6~I~O~I~4;-lc8~2c2J-T2~6~~1cO'~I>'~18~~22~ o Fractions Fig. 6A-D. The profiles of polyribosomes extracted from permissive (HEp-2) and nonpermissive (DK) cells infected with the MPdk- and MPdk+sp mutants. Extracts of labeled cells were mixed and co-centrifuged with an extract of an excess of unlabeled cells. The absorbance monitored after centrifugation was due mostly to unlabeled extract. A Artificial mixture of extracts of HEp-2 cells infected with MPdk- virus and labeled with 3H amino acid, DK cells infected with the same virus and labeled with 14C amino acids, and unlabeled HEp-2 cells infected with the same virus.
The paper did not show whether the RNA complementary to viral DNA was the one accepting the amino acid. It is conceivable that small fragments of labeled viral mRNA could have eluted together with unlabeled host sRNA synthesized before infection. Two recent experiments are more convincing. In the first, SUBAK-SHARPE, SHEPHARD and HAY (1966) showed partial separation of arginyl-sRNA from infected and unifected cells. In the second they digested with ribonuclease T 1 artificial mixtures of 3H labeled aminoacyl-sRNA from uninfected cells and 14C labeled amino-acyl-sRNA infected cells.
1. T. in Microbiology, Vol. 49 50 B. ROIZMAN: it seems puzzling that DNA viruses carry such information and expend the host's energy to synthesize more molecules concerned with the same function. The question is particularly appropriate if one considers that, while the doubling time of most animal cells is nearly equal to the reproductive cycle of DNA viruses, the amount of cellular DNA and protein synthesized by the cell greatly exceeds the amount of viral DNA and protein made during the same period (KAPLAN and BEN-PORAT, 1960, 1966; SYDISKIS and ROIZMAN, 1966).
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