By Joanna B. Goldberg

Bacterial floor or secreted polysaccharides are molecules that may functionality as limitations to guard bacterial cells opposed to environmental stresses, in addition to act as adhesins or acceptance molecules. every so often, those molecules are immunodominant antigens eliciting a lively immune reaction, whereas in different circumstances the expression of polysaccharides camouflages the micro organism from the immune method. till lately, such a lot reviews at the enzymatic steps and law of those molecules have been played at the enteric gram detrimental micro organism Escherichia coli and Salmonella typhimurium. With the arrival of contemporary bacterial genetics, thoughts comparable to building and characterization of polysaccharide mutants, cloning of genes and complementation of those mutations, and expression of polysaccharides in heterologous bacterial hosts has caused investigations into the jobs and capabilities of those molecules for lots of various bacteria.Here, we current the genetic research of polysaccharides from a few micro organism pathogenic to people and one symbiotic with crops in hopes that similarities within the experimental methods in addition to discovering s from such investigations could lead on to a common realizing of polysaccharide synthesis and legislation in a number of micro organism. good points

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When we started to study the O-antigen genetics in Y. enterocolitica O:8,26 our first intention was to isolate rough mutants using an in vivo chromosomal transposon mutagenesis. 26 A number of mutants were obtained and analyzed for LPS patterns by SDS–PAGE analysis. All the mutants were shown to be devoid of O-antigen, and only expressed LPS molecules containing lipid A and a core oligosaccharide which at that time, based on the estimated size, were erroneously regarded as truncated. 36 The clones expressing the O:8 O-antigen were screened using specific Mab.

0021/ch01/frame Page 21 Thursday, September 6, 2007 2:07 PM Chapter one: Genetics of Pseudomonas aeruginosa polysaccharides 21 80. Wyckoff, T. J. , Raetz, C. R. , and Jackman, J. E. Antibacterial and antiinflammatory agents that target endotoxin, Trends in Microbiology, 6, 154-159, 1998. 81. Onishi, H. , Pelak, B. , Gerckens, L. , Silver, L. , Kahan, F. , Patchett, A. , Galloway, S. , Hyland, S. , Anderson, M. , and Raetz, C. R. H. Antibacterial agents that inhibit lipid A biosynthesis, Science, 274, 980-982, 1996.

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Genetics of Bacterial Polysaccharides by Joanna B. Goldberg
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