By Kiran Kapoor

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The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Animal cell culture became a common laboratory technique in the mid-1900s, but the concept of maintaining live cell lines separated from their original tissue source was discovered in the 19th century The 19th-century English physiologist Sydney Ringer developed salt solutions containing the chlorides of sodium, potassium, calcium and magnesium suitable for maintaining the beating of an isolated animal heart outside of the body.

Nitrate tests are negative. Bacillus coagulans has been added by the EFSA to their Qualified Presumption of Safety (QPS) list and has been approved for veterinary purposes as GRAS by the FDA's Center for Veterinary Medicine, as well as by the EU and is listed by AAFCO for use as a direct fed microbial in livestock production. Its main use is thus is veterinary applications, especially as a probiotic in pigs and shrimp. There are also references to use of this bacterium in humans, especially in improving the vaginal flora, improving abdominal pain and bloating in IBS patients and increasing immune response to viral challenges.

B. anthracis was the first bacterium conclusively demonstrated to cause disease, by Robert Koch in 1877. The species name anthracis is from the Greek anthrakis, meaning coal and referring to the most common form of the disease, cutaneous anthrax, in which large black skin lesions are formed. 24 Illustrated Dictionary of Microbiology Bacillus coagulans : It is a lactic acid forming bacterial species within the genus Bacillus. The organism was first isolated and described in 1932 and was elaborated in the fifth edition of Bergey's Manual of Determinative Bacteriology.

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Illustrated dictionary of microbiology by Kiran Kapoor
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