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B. Effect on metabolic activities . Control of Temperature in Microbiological Studies . 11. A. Maintenance of stock cultures B. Incubation of experimental cultures . C. Harvesting and handling of suspensions . D. Methods used to study metabolic activities . E. Temperature measurement in natural environments References . . 23 23 25 25 25 26 31 32 37 37 I. EFFECTS OF TEMPERATURE ON MICRO-ORGANISMS A. Effect on growth T h e pioneer microbiologists were quick to realize that the rate of growth of a microbe is affected by the environmental temperature, and several studies on the effect of temperature on growth of pure cultures of microorganisms were made during the late nineteenth century.
And Rose, A. H. J. , 98, in press. , and I h n t z , J. A. (1954). Ann. N . Y . Acud. , 58,1303-1310. 38 J. W. PATCHING AND A. H. , and Wallach, D. F. H. (1968). In “Biological Membranes” (Ed, D. Chapman), pp. 125-202. Academic Press, London. Collins, C. H. (1967). “MicrobiologicalMethods”, p. 53. Butterworths, London. Deutsch, W. W. (1962). Rev. scient. , 33, 249-250. Dimmick, R. L. (1965). Appl. , 13, 846-850. Elliott, R. , and Heiniger, P. K. (1965). Appl. , 13, 73-76. , and Rose, A. H. (1967a).
While the optimum, maximum and minimum temperatures for growth are considered to be cardinal by most microbiologists, it is as well to remember that the values for these temperatures, for any one organism, may vary depending upon the chemical and physical properties of the environment. Despite these reservations concerning the cardinal temperatures for growth, microbiologists have continued to use them to separate microbes into the three categories known as psychrophiles, mesophiles and thermophiles.
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