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The significantly acclaimed laboratory commonplace, Methods in Enzymology, is without doubt one of the so much hugely revered courses within the box of biochemistry. on the grounds that 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate today--truly a vital book for researchers in all fields of lifestyles sciences
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Extra resources for Proteolytic Enzymes in Coagulation, Fibrinolysis, and Complement Activation Part A: Mammalian Blood Coagulation Factors and Inhibitors
Banyai,A. Varadi, and L. Patthy, FEBSLett. 163, 37 (1983). ~oL. Patthy,M. Trexler,Zs. Vail, L. Banyai,and A. Varadi,FEBS Lett. 171, 131 (1984). H R. F. Doolittle, D. F. Feng,and M. S. Johnson, Nature (London) 307, 558 (1984). n L. ) 41, 657 (1985). ~3L. Patthy,Semin. Thromb. Hemostasis 16, 245 (1990). ~4L. Patthy, Blood Coagulation Fibrinolysis 1, 153 (1990). ~sL. Patthy,Curr. Opin. Struct. Biol. 1, 351 (1991). [ 1] MODULAR DESIGN OF PROTEASES 15 interactions changing regulation, specificity, and targeting of proteases are desirable.
Haggroth, and L. , Fed. Am. Soc. Exp. Biol. 40, 1585, Abstr. 258 (1981). 34 L. Lorand and T. Gotoh, this series, Vol. 19, p. 770. 37 C. G. Curtis and L. Lorand, this series, Vol. 45, p. 177. 3s O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. Randall, J. Biol. Chem. 193, 265 0951). 3, 26 M A M M A L I ACOAGULATION N FACTORS AND INHIBITORS A I B 2 3  C t 2 3 4 5 6 7 FIG. 1. Association of the recombinant protein rA2 with its native B2 carrier from plasma, studied by nondenaturing electrophoresis.
5% SDS, and are heated to 100 ° for 10 rain prior to application to the gel. Electrophoretic separations are carried out at about 10 mA (70 V-hr). The gel is silver stained for protein using the photochemical procedure of Oaldey et al. 39 and the Bio-Rad Silver Stain Kit (Bio-Rad, Richmond, CA). The gel filtration data are presented in Fig. 2, and these, too, prove the high affinity of rA2 for B2. 2 pmol, shown in 39B. R. Oakley,D. R. Kitsch,and N. R. Morris,Anal. 105, 361 (1980). 28 MAMMALIAN COAGULATION FACTORS AND INHIBITORS [I-)] ~r tt) O o4 CO o4 o4 1= 2' 5' 6' o4 94 68 L6 i _J~ ,,¢¢ 43 o4 I 3 I ~ -2o 4 p,..
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